Image cytometry-based detection of aneuploidy by fluorescence in situ hybridization in suspension
نویسندگان
چکیده
منابع مشابه
Detection of aneuploidy in human spermatozoa of normal semen donors by fluorescence in situ hybridization.
We have studied human spermatozoa from 24 normal, healthy unexposed men, 18 of whom were semen donors at the Sperm Bank in Turku, using multicolor fluorescence in situ hybridization with two chromosome-specific probes. The possible age-related increase in aneuploidy frequencies was assessed. Ten thousand spermatozoa were scored per individual for the presence of hyperploid, i.e., disomic and di...
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Fluorescence in situ hybridization (FISH) is becoming increasingly used to detect chromosomal changes in cancer cytogenetics. Here, we report its use in human HL60 cells to detect aneuploidy induced by the benzene metabolite, 1,2,4-benzenetriol (BT). Human centromeric probes specific for chromosomes 9 and 7 were used. Untreated HL60 cells were 0.72 +/- 0.29% hyperdiploid for chromosome 9. Treat...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2012
ISSN: 1552-4922
DOI: 10.1002/cyto.a.22101